Research platform on antibiotic resistance spread through wastewater treatment plants

Newsletter #2
November 2020                                                

Dear colleagues,

we are pleased to send you the second newsletter of REPARES. As in the previous one, we introduce to you the activities of the project that was going forward even in the difficult conditions of the COVID crisis. We conducted an inter-laboratory study that is the first step towards a standardized methodology for ARG detection in wastewater and sewage sludges. We are also excited to announce the widening of the consortium by a new partner - the University of Warsaw (UW), more specifically the group of professor Lukasz Dziewit, who will strengthen the team with their great expertise in bioinformatics and the biology of mobile genetic elements of bacteria. Last, but not least, we again bring a selection of recent advances in ARG/ARB related research.

We wish you inspiring reading and good nerves in the difficult COVID times.

Jan Bartacek

Principal Investigator of REPARES
University of Chemistry and Technology Prague

University of Warsaw          

News in ARG research

Renzo Dukker from TU Delft finished his BSc thesis on the impact of DNA extractions on microbial community and antimicrobial resistance profiling from activated sludge. His work directly follows up on the REPARES wetlab seminar organized in TU Delft in January 2020. Congratulations! 

The group of REPARES scientists from UCT Prague received a new project on ARG and COVID in WWTPs in the Czech Republic. Project Technology for removal of antibiotic resistance genes from sewage sludge applied in agriculture (ARG Tech) is for 3 years and its main goal is to design the process of sludge sanitation with successful ARB and ARG removal to use the sludge as an agricultural fertilizer.

Metagenomic analysis of an urban resistome before and after wastewater treatment, Felipe Lira, Ivone Vaz-Moreira, Javier Tamames, Célia M. Manaia & José Luis Martínez (Science Report 10, 8174, 2020) (contribution of REPARES members)
Persistence of wastewater antibiotic-resistant bacteria and their genes in human fecal material, Nazareno Scaccia, Ivone Vaz-Moreira, Célia M Manaia (FEMS Microbiology Ecology, Volume 96, Issue 6, June 2020) (contribution of REPARES members)
Revealing antimicrobial resistance in stormwater with MinION, Maciej Białasek, Aleksandra Miłobędzka (Chemosphere, Volume 258, November 2020) (contribution of REPARES members)
Conventional and emerging technologies for removal of antibiotics from wastewater, Bao Lee Phoon et al. (Journal of Hazardous Materials, Volume 400, 5 December 2020)
Antibiotic resistance genes and mobile genetic elements removal from 2 treated wastewater by sewage-sludge biochar and iron-oxide coated sand, David Calderón-Franco, Apoorva Seeram, Gertjan Medema, Mark C. M. van Loosdrecht, David G. Weissbrodt (bioRxiv preprint) (contribution of REPARES consortium member)

Sabina Purkrtová (UCT Prague)
Sabina Purkrtova is presently an assistant professor in the Department of Biochemistry and Microbiology UCT Prague. From 2008 she is a team-member of the Testing Laboratory of the Department of Biochemistry and Microbiology No. 1316.3 ...
David Calderón Franco (TU Delft)
David Calderón Franco studied the BSc in Biotechnology at the Autonomous University of Barcelona, Spain, and then pursued an MSc in Bioengineering at the Institut Químic de Sarrià ...
Ivone Vaz-Moreira (UCP)
Ivone Vaz-Moreira is a researcher at CBQF (Centre of Biotechnology and Fine Chemistry), at the Portuguese Catholic University, where she also teaches in the areas of Bioinformatics and Biochemistry ...
Marta Nierychlo (AAU)
Marta Nierychlo is a member of the Center of Microbial Communities at Aalborg University, Denmark. She obtained her Ph.D. degree in biotechnology in 2013, and since then is working in the group of prof. Per Halkjær Nielsen ...
Lucia Hernandez-Leal (Wetsus)
Dr. Lucia Hernandez-Leal has more than 15 years of research experience on wastewater treatment, in particular on source-separated sanitation, removal of micropollutants, and more recently antimicrobial resistance in the water cycle ...
Lukasz Dziewit (WU)
Doctor Lukasz Dziewit represents the University of Warsaw, Poland. He is a co-founder of Drewniak and Dziewit group and a director of the Department of Environmental Microbiology and Biotechnology ... 
Meetings & Research visits

Second workshop on Methodologies for antibiotic resistance monitoring

taking place in Prague, Czech Republic, on July 23rd, 2020, was hosted by Jan Bartacek and Aleksandra Milobedzka ...

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REPARES in the time of COVID-19 

Covid 19 pandemic severely influenced the activities planned in REPARES as travelling between partner universities is, indeed, a vital component of the project. We could not organize ...

Read more


REPARES Future plans & actions
  • On November 18, 2020, at 10:00 CET, prof. Celia M. Manaia.will give an online lecture regarding Antibiotic resistance "Antibiotic Resistance in the Environment: Why it is important?". To register for this lecture, please send an email to with the subject of the email "Registration for online ARG presentation". More information about the lecture and lecturer can be found here.
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